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Sunday, July 5, 2020 | History

2 edition of Studies on DNA synthesis in cells affected with polyoma virus. found in the catalog.

Studies on DNA synthesis in cells affected with polyoma virus.

Philip Edward Branton

Studies on DNA synthesis in cells affected with polyoma virus.

by Philip Edward Branton

  • 381 Want to read
  • 16 Currently reading

Published in [Toronto] .
Written in English

    Subjects:
  • Deoxyribonucleic acid,
  • Polyomaviruses,
  • Virus research

  • Edition Notes

    ContributionsToronto, University.
    The Physical Object
    Pagination164, [22] leaves.
    Number of Pages164
    ID Numbers
    Open LibraryOL21625638M

    The large tumor antigen plays a key role in regulating the viral life cycle by binding to the viral origin of DNA replication where it promotes DNA synthesis. Also as the polyomavirus relies on the host cell machinery to replicate the host cell needs to be in s-phase for this to : incertae sedis. B) dsRNA viruses quickly transcribe their genes into mRNA which is insensitive to immune responses. C) genomes of RNA viruses are often chemically modified to avoid recognition by human immune cells. D) the genomes of dsRNA viruses must avoid human immune cells during infection, including replicating their genomes within their own nucleocapsids.

    Tumor DNA viruses enhance aerobic glycolysis upon virus-induced cell transformation, supporting rapid cell proliferation and showing the Warburg e ect. Moreover, viral proteins enhance glucose uptake and controls tumor microenvironment, promoting metastasizing of the tumor cells. 1. Introduction Development of cancer is a multistep process. Viruses & DNA + Protein Synthesis study guide by rghuman includes 34 questions covering vocabulary, terms and more. Quizlet flashcards, activities and games help you improve your grades.

      In marked contrast to simian virus 40 (SV40), polyoma virus (PyV) has been reported to replicate discontinuously on both arms of replication forks. In an effort to clarify the relationship between the mechanisms of DNA replication in these closely related viruses, the distribution of RNA-primed DNA chains at replication forks was examined Cited by: Human polyomavirus 2, commonly referred to as the JC virus or John Cunningham virus, is a type of human polyomavirus (formerly known as papovavirus). It was identified by electron microscopy in by ZuRhein and Chou, and by Silverman and Rubinstein, and later isolated in culture and named using the two initials of a patient, John Cunningham, with progressive multifocal leukoencephalopathy Class: incertae sedis.


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Studies on DNA synthesis in cells affected with polyoma virus by Philip Edward Branton Download PDF EPUB FB2

The formation of viral DNA was inhibited in polyoma virus-infected cells in which protein synthesis had been blocked by cycloheximide. The present studies show the following.

(i) The pool of replicating viral DNA molecules was reduced in cycloheximide-treated Cited by: 9. It had previously been established that cell DNA synthesis is inhibited in growing mouse embryo cells productively infected at high multiplicity with Cited by: In virus-infected ts A1S9 cells incubated at C, cell DNA synthesis appears to proceed almost entirely by a process analogous to repair replication.

The inability of ts A1S9 cells to produce large-molecular-weight chromosomal DNA strands, at C, by the normal mechanism is not overcome by Py by: The transformation of normal cells into tumor cells by polyoma virus is caused by the interaction of susceptible cells with the DNA of the virus.

Thus, purified polyoma virus DNA has been shown to transform cells cultured in vitro, 1 whereas the empty protein shells of the virus do not produce this effect.

2 Consequently, a knowledge of the functions of the viral genes is basic to an understanding of the mechanisms of cell transformation Cited by: Confluent mouse embryo monolayer cultures are induced by infection with polyoma virus to synthesize DNA which behaves on columns of methylated albumin Cited by: Branton PE, Sheinin R.

Control of DNA synthesis in cells infected with polyoma virus. Virology. Dec; 36 (4)– Butler WB, Mueller GC. Control of histone synthesis in HeLa cells. Biochim Biophys Acta. Feb 4; (1)– Cheevers WP. Protein and messenger RNA requirements for superhelicity of polyoma virus DNA.

Abstract The induction of DNA polymerase activity and of DNA synthesis in polyoma virus-infected mouse kidney cells is inhibited by actinomycin D and cycloheximide, even in cells that are already T-antigen positive. This indicates that the induction of the DNA-synthesizing apparatus requires transcription and by: 9.

The DNA of polyoma virus migrates much faster than host cell DNA (native or denatured) on electrophoresis in agar gel. This finding provides a simple means by which the synthesis of viral and cellular DNA can be examined quantitatively in infected by: Polyoma virus has three late mRNA's: one for each virion protein.

J Virol. Aug; 27 (2)– [PMC free article] Smith AE, Kamen R, Mangel WF, Shure H, Wheeler T. Location of the sequences coding for capsid proteins VP1 and VP2 on polyoma virus DNA. Cell. Nov; 9 (3)– Soeda E, Arrand JR, Griffin BE. Polyoma by: BK polyomavirus (BKPyV) is a small double-stranded DNA virus that is an emerging pathogen in immunocompromised individuals.

BKPyV is widespread in the general population, but primarily causes disease when immune suppression leads to reactivation of latent by: INTRODUCTION In a previous study it was shown that early protein appeared in polyoma virus (PV)-infected mouse embryo cells at hours after infection, and that viral DNA synthesis commenced about ') hours after early protein synthesis (Gershon and Sachs, ).Cited by: 4.

Summary Various mammalian cell cultures were examined for their ability to support polyoma virus DNA replication, and for induction of cellular DNA synthesis, as a result of infection by polyoma by: 1.

The data obtained in the present study lead to the conclusion that polyoma virus is capable of initiating mitosis independent of the induction of DNA synthesis. This sug- gests that viruses may affect cells withdrawn from the mitotic cycle at different by: 2. The activation of the human polyomavirus BK causes polyomavirus-associated nephropathy in immunocompromised humans.

Studies of the virus have been restricted since the virus DNA replication is. Polyoma virus DNA detection and subsequent differentiation between BK and JC virus. subjects is essentially asymptomatic with the virus persisting thereafter in the kidneys and peripheral white blood cells.

Reactivation of the virus with shedding in the urine occurs frequently in the immunosuppressed and has a low predictive value for BK. Polyomavirus replication is heavily reliant on the host DNA synthesis machinery, and it has previously been shown that either BKPyV infection or JC polyomavirus (JCPyV) LTAg expression.

In the present study, we used BrdU pulse labeling to demonstrate that PML-NBs accumulate newly synthesized DNA in cells infected by the polyomaviruses simian virus 40 (SV40) or polyomavirus. Summary. In this chapter we review studies on the organization and replication of polyoma virus (Py) DNA sequences which have integrated into chromosomal DNA, and of cellular DNA flanking the Py integration site, in an inducible line of polyoma-transformed rat cells designated the LPT by: 1.

The BK virus is a member of the polyomavirus family. Past infection with the BK virus is widespread, but significant consequences of infection are uncommon, with the exception of the immunocompromised and the virus is an abbreviation of the name of the first patient whom the virus was isolated from in (the patient was then 29 years old).Specialty: Infectious disease.

Previous studies in the small DNA virus polyomavirus (PyV) family demonstrated that PyV miRNAs directly regulate early viral transcripts [19, 54,55]. Further, bandicoot papillomatosis. We have produced nonviable deletion mutants of polyoma virus in order to study homologous recombination after DNA transfection into mouse cells.

The frequency of recombination was determined by the formation of infectious virus. It was dependent on the amount of DNA transfected and the size of the region of homology between the by: 4.THE JOURNAL OF BIOLOGICAL CHEMISTRY (c> by The American Society for Biochemistry and Molecular Biology, Inc.

Vol.No. 19, Issue of July 5, pp.Printed in U. S.A. DNA Helicase and Duplex DNA Fragment Unwinding Activities of Polyoma and Simian Virus 40 Large T Antigen Display Similarities and Differences*.Polyomavirus BK (BKV) is small double stranded DNA virus that has been assigned to the Polyomaviridae family.

The BK virus genome consists of a non-coding control region National Coalition for Cancer Research (NCCR), the early coding region that transcribes the T antigen, and the late coding region, which codes for the viral capsid proteins (VP-1, VP-2, VP-3) and agnoprotein ().